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Report for SNP rs679620

Developed by Shray Alag, 2020.
SNP Clinical Trial Gene

There is one clinical trial.

Clinical Trials


1 Design of a Protocol for Obtaining Genomic DNA From Saliva Using Mouthwash: Samples Taken From Patients With Periodontal Disease

Objective The aim of this study was to test a protocol for the extraction of high quality genomic DNA from saliva samples obtained with mouthwash and taken from patients with periodontal disease. Materials and methods Saliva samples were taken from 60 patients, then stored at room temperature. DNA extraction was carried out at distinct post-sampling times (10, 20 and 30 days). Evaluation of genomic DNA was performed with spectrophotometry, electrophoresis, and PCR genotyping and sequencing.

NCT02523326 Periodontal Disease Periodontal Disease " Periodontal Disease "Unrecognized Condition Periodontal Disease "Unrecognized Condition" Device: saliva samples obtained with mouthwash
MeSH: Periodontal Diseases
HPO: Periodontitis

To verify the quality and species (human) of the DNA extracted, primers were designed for the rs679620 polymorphism (forward 5´- ggg gCT TAA ggC ACA TgA gT-3; reverse 5´- ACT TCg ggA TgC CAg gAA Ag - 3´; Tm: 56°C, 40 cycles) that amplified a product of 485 bp.

Primary Outcomes

Description: high quality genomic DNA from saliva samples obtained with mouthwash.

Measure: DNA

Time: 10 days [group A], 20 days [group B], 30 days [group C]


HPO Nodes


Periodontitis
Genes 41
PLG GORAB PLG DKC1 FGF3 CTSC CTSC AP3B1 SLC35C1 ELANE NOTCH2 CTSC PLG RTEL1 CXCR4 TERC PARN GFI1 TCIRG1 FERMT1 OCRL ELANE C1S SRP54 ITGB2 C1R USB1 CTC1 LYST NOP10 COL3A1 NPM1 WRAP53 C1S TERT COL3A1 C1R NHP2 CTSC TINF2 FERMT1