1. The polycystic ovary syndrome is the major cause of infertility in the United States. Metformin has been shown to increase frequency of ovulations in PCOS, and is used in clinical practice to treat infertility, but some women with PCOS do not respond to metformin treatment. 2. Knowing that a specific gene predicts the effect of metformin on ovulation would facilitate more efficient and effective treatment of infertility in PCOS.
Name: Metformin 500 mg tabletDescription: Metformin 500 mg tablets; two tablets every 12 hours for 9 monthsType: Drug
Description: Responders were defined as those that had a doubling of baseline ovulation rate estimated by self-report of menstrual history.Measure: Number of Responders/Non-responders for Each STK11 rs8111699 Genotype (C/G, C/C, G/G) Time: 9 months
Description: Ovulations were determined by measurement of daily urine pregnanediol-3-glucuronide or weekly progesterone levels over 6-9 months of study duration for each participant. The ovulation rate was calculated as the number of confirmed ovulation events per months of study participation.Measure: Ovulation Rate Over Study Duration for STK11 Genotypes CC, CG and GG Time: 9 months
Description: Bivariate fit (RSquare with P values) of ovulation rate post treatment by change in total testosterone and Matsuda Index for each of the 3 genotypes (G/G, C/G, C/C)Measure: Determine in Which Genotype(s) Frequency of Ovulation Correlates With Improvement in Reduction in Total Testosterone and Insulin Sensitivity as Measured by the Matsuda Index. Time: 9 months
Single Group Assignment
There is one SNP
Number of Responders/Non-responders for Each STK11 rs8111699 Genotype (C/G, C/C, G/G).
We hypothesize that women with the polycystic ovary syndrome (PCOS) who have the G/G genotype of single nucleotide polymorphism (SNP)_ rs8111699 in STK11 will exhibit a significantly greater response to metformin, in terms of ovulation, compared with women with either the C/G or C/C genotype.